Eradication of the cattle tick “Boophilus annulatus" was achieved by using the tick decoy technology. The identification and analysis of the female tick pheromone composition were carried out using thin layer chromatograph (TLC) and high pressure liquid chromatography (HPLC). Bioassays were done to determine the B.annulatus male responses to the prepared B.annulatus female pheromone. The results showed high male responses (56.66%). Application of manufactured pheromone – pyrethroid decoys of the male B.annulatus ticks gave great mortality of ticks ( 83.33%) . A field trial to evaluate the efficiency of tick decoys coated with Boophilus annulatus pheromones was done on eight cows.Decoys containing 10% ectomin were used. Each cow was naturally infested with 25 female and 25 male Boophilus annulatus ticks. Fifty pheromone ectomin impregnated decoys were glued in the predilection seats to each cow of the experimental group. Following decoys attachment, male ticks began to detach, attracted to the decoys and died with mortality rate reached 76%. Female ticks after incubation laid unfertile eggs.  Key words = Boophilus annulatus, pheromone , Decoys , Thin layer chromatography (TLC) and High pressure liquid chromatography (HPLC) .