This study was performed to investigate the prevalence of Cysticercus bovis infection in both cattle and buffaloes in Beni-Suef Governorate. For this purpose, a total number of 2156 cattle and buffaloes (1313 cattle and 843 buffaloes) of different ages were examined in Beni-Suef abattoir. Among those, 28 animals (cattle and buffaloes) were infected with C. bovis with a percentage of 1.3%. Among cattle, 21 animals were infected with a percentage of 1.6%, while 7 buffaloes of different ages were infected with a percentage of 0.83%. Moreover, we found that infection rate was affected by aging i.e. among 1005 cattle calves of upto 2 years, only 13 were positive for cysticercosis by meat inspection with a percentage of 1.29%, while among 308 old-aged cattle of at least 5 years, 8 cows were infected with a percentage of 2.6%. On the other hand, examination of 695 buffalo calves of upto 2 years revealed 5 calves infected with C. bovis with a percentage of 0.72%, while old-aged buffaloes of more than 5 years showed 2 affected animals with a percentage of 1.35%. Besides, application of ELISA revealed positive results for all cases showed C. bovis by meat inspection. Moreover, results of Western blot showed 2 bands of molecular weights 31 kDa and 18 kDa respectively found in all positive cases for cysticercosis, so they are considered as highly diagnostic bands for diagnosis of bovine cysticercosis. Also, 2 bands of molecular weights 47.5 kDa and 65 kDa were found in 60% of positive cases. Therefore, serological tools should be used parallel with routine meat inspection in abattoirs in order to obtain an appropriate data on bovine cysticercosis and so we can reach the proper means of control.