A total of 260 of raw milk samples were collected from different localities in Assiut Governorate. These samples represented by 210 and 50 each of raw milk as well as milk whey samples obtained from cows and buffaloes, respectively. The incidence of brucella antibodies in milk samples were estimated by MRT and by wRBPT, wBAPAT, wRiv.T and wTAT in their corresponding whey samples. Out of 210 cows milk samples examined by MRT, 12.38% were positive (constituting 4.76, 2.38 and 5.24% were positive in grade (++), (+++) and (++++), respectively), 7.62% were doubtful and 80% were negative. In the corresponding milk whey samples by whey serological tests: wRBPT, wBAPAT, wRiv.T and wTAT gave 4.29, 4.29, 4.29 and 5.24% positive, while, the negative results were 95.71, 95.71, 95.71 and 94.76%, respectively. In case of buffalo's milk, all of the examined milk as well as milk whey samples found to be negative to MRT as well as to all whey serological tests.