In this study, a total of 165 calves (125 diarrhoeic and 40 healthy calves) were examined for bacteriological and mycological isolation to study the role of family Enterobacteriaceae and fungi in calf diarrhoea. A total of 314 bacterial isolates were recovered from examined calves and identified into E. coli (66.2%), P. mirabilis (12.1%), Citrobacter spp. (12.1%), Enterobacter spp. (3.2%), Klebsiella spp. (2.2%), P. vulgaris (1.6%), Salmonella spp. (0.9%), Edwardsiella spp. (0.6%), Morganella spp. (0.6%) and Serratia spp. (0.3%). Salmonella spp. had been recovered only from diarrhoeic calves. The recovered isolates were S. kentucky, S. ferruch and S. cerro (0.3% of each). Congo red binding activity of E. coli may be considered as an easy diagnostic tool for rough differentiation between pathogenic and non pathogenic strains. A total of 12 yeast isolates were recovered from examined calves and identified into Candida glabrata (58.3%), Candida guillermondii (33.3%) and other Candida spp. (8.33%). In vitro sensitivity of E. coli to various antimicrobial agents revealed their sensitivity to ciprofloacin, enrofloaxin, ofloxacin and chloramphenicol, while they were resistant to oxytetracycline, colistine, clindamycine and erythromycine, on the other side, Salmonella spp. were varied in their sensitivity to different antimicrobial agents.