One hundred and fifty random samples of raw milk from cows, Buffaloes and Sheep (50 samples of each) were collected from different farmer's houses and dairy shops in Assiut Governorate to be examined for the presence of salmonella organisms on pre – enrichment and enrichment then plating on selective agar media, the Biochemical tests and serological tests were applied. The obtained results revealed that, the salmonella Spp. could be detected from raw milk of different animals were 25 (6.17%) positive samples, 1 (2%), 10 (20%) and 14 (28%) for cows, buffaloes and sheep milk, respectively. Serotyping of the isolated salmonella spp. revealed that, the Salmonella gallinarum was the most prevalent species among the salmonalla spp. 15 isolates (10%) 10 (20%) from buffaloes and 5 (10%) from sheep. Salmonella enteritidis was isolated from 9 samples, 1 (2%) from cows and 8 (16%) from sheep but only one sample of sheep milk was positive for salmonella typhi. All types of the isolated salmonella were highly sensitive to Norofloxacin of the tested antiblotics and moderately sensitive to streptomycin and weekly sensitive to Gentamycin, Rifampin, Ampicillin, Cefotaxime, cefadroxil and chloramphenicol. However salmonella gallinerum was moderately sensitive to Gentamycin and resistant to Cefadroxil and Chloramphenical.