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DIETARY ADMINISTRATION OF UNDENATURED WHEY PROTEIN DECREASED THE HISTOPATHOLOGICAL CHANGES INDUCED BY CIPROFLOXACIN IN HEPATIC, RENAL AND ILAEL TISSUES OF MICE

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Last updated: 23 Jan 2023

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Abstract

Whey protein contains all amino acids that are necessary for normal cell growth. This work aimed to study whether whey protein can decrease the effects induced by Ciprofloxacin. Histological, histochemical and immunological methods were used. Mice were classified into a control (group I) and three treated groups. Group II was daily administrated Ciprofloxacin for 7 days. Group III was administrated Ciprofloxacin and synergistically mice administrated whey protein for the 7 days. Mice of group IV were treated in the same way like as those of group III with the whey protein continue for 8 days more (whey protein was administrated 15 days in group IV instead of 7 days in group III). Liver sections of group II revealed that cell boundaries of hepatocytes became unclear, blood sinusoids became narrow and Kupffer cells increased in both size and number. PAS stain showed a reduced carbohydrate content in group II, while it showed a little improved in group IV. A moderate improvement in the protein content was observed in this group. Kidney sections of group II appeared with some cellular inflammations, edematous renal cells and glomeruli were shrunken. Also a decrease in carbohydrate and protein contents was observed. An obvious effect of the whey protein had obtained of the renal tissues in group IV, since glumeruli, renal tubules and the general histological architecture became similar to the normal. Ileum sections revealed a thickness in the muscularis and increasing in crypts of Lieberkuhn and in fibers. An improvement was noted in group III and increased as the time of whey protein prolonged in group IV. There was an observed decrease of the carbohydrate contents in both group II and group III. This carbohydrate contents and intensity was obviously increased in goblet cells and brush border of group IV. The intensity of proteins in the tissues of treated groups was nearly as that in control. The physiological and immunological indices reflected the same severe effect of the antibiotic. An observed improvement was achieved in the phagocytic activity and the chemokinetic index after administration of the undenatured whey protein. In conclusion, these findings showed severe effects of the Ciprofloxacin on the histological architecture of studied organs. After 15 days (group IV) an improvement was observed in both renal and ileal tissues but could not completely restore their normal histological architecture. This protein obviously affected the physiological and the immunological responses, and this might be the potential factor in initiating the normalizing processes of the histological architecture structures. 
 
Key words: Undenatured whey protein, Ciprofloxacin, histopathological changes, Immunological response, mice.
 
Introduction
 
Undenatured whey protein has been found to have a number of bioactive properties. Normal processes to extract whey component from the other constituents lead to significant denaturing of the bioactive whey proteins. Undenatured whey protein isolates utilize proprietary processes to attain a protein containing over 90% undenatured whey protein. Bioactive components include lactoferrin, lysozyme, lactoperoxidase, glycomacropeptide, alpha-lactalbumin, bovine serum albumin, various growth factors and immunoglobulins. Lactoferrin exhibits anticancer, antiviral, antibacterial, and antifungal activity. It plays active roles in iron transport and in the cytotoxic defenses of neutrophils, and it scavenges free iron which acts as a free radical (Barta et al., 1991; Kawasaki 1993; Marchetti 1994; Wong and Watson 1995).
Whey protein is believed to be the highest quality protein available when compared to different proteins. It contains all essential and non-essential amino acids and is a good source of glutamine and the branched-chain amino acids that are necessary for cell growth (David 1999). These amino acids include arginine, glycine, leucine, isoleucine and valine which are essential to different tissues (Ganong 1997). The present work aimed to investigate the effect of the undenatured whey protein on decreasing the harmful effects of the antibiotic, Ciprofloxacin on the histological structure of some tissues and some immune parameters.

DOI

10.21608/avmj.2008.174659

Keywords

DIETARY ADMINISTRATION OF UNDENATURED WHEY PROTEIN DECREASED THE HISTOPATHOLOGICAL CHANGES INDUCED BY CIPROFLOXACIN IN HEPATIC, RENAL AND ILAEL TISSUES OF MICE

Authors

First Name

MANAL

Last Name

BAYOMI

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Affiliation

Dept. of Zoology, Faculty of Science, El-Minia University.

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Orcid

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First Name

H.

Last Name

EBAID

MiddleName

-

Affiliation

Dept. of Zoology, Faculty of Science, El-Minia University.

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City

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Orcid

-

First Name

KARIMA

Last Name

HASSANEIN

MiddleName

-

Affiliation

Dept. of Dairy, Faculty of Agriculture, El-Minia University.

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Volume

54

Article Issue

116

Related Issue

24388

Issue Date

2008-01-01

Receive Date

2007-12-13

Publish Date

2008-01-01

Page Start

1

Page End

25

Print ISSN

1012-5973

Online ISSN

2314-5226

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https://avmj.journals.ekb.eg/article_174659.html

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https://avmj.journals.ekb.eg/service?article_code=174659

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4

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Journal

Publication Title

Assiut Veterinary Medical Journal

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https://avmj.journals.ekb.eg/

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Created At

23 Jan 2023