This study was carried out on 60 ovarian samples of normal cyclic non-pregnant buffalo-cows. The experimental period extended for eight months (four months for each of dry and green seasons). The samples were collected from 60 normal cyclic non – pregnant slaughtered buffalo – cows. (30 samples during the dry seasons and 30 samples during the green one). The age of the animals was ranging between 4 – 8 years old. This study was carried out to test possible the variations between some hormonal and biochemical constituents of the follicular fluid during these different seasons in normal non – pregnant cyclic. Buffalo - cows which were tested during proestrus and oestrus phases and samples were collected after slaughtering and evisceration. The study included the effect of nutrition on the determined parameters of oestrogens, progesterone, total protein, albumen, globulin, AST, ALT, inorganic phosphorus, calcium manganese, iron, Zinc and copper. The results revealed that, there was a high significant increase in both of follicular oeotrogens and progesterone during the green season than the dry one. A high significant increase in the level of total protein and albumin during the green season than the dry one. Also a non significant increase in globulin level during the green season than the dry one. The study recorded also a significant increase in transminases AST and ALT levels during the dry seasons than the green one. The results revealed a high significant increase in inorganic phosphorus level and only a significant increase in calcium level during the dry season than the green one. This study showed also a significant increase in manganese and a nonsignificant increases in zinc and copper in the follicular fluid of the buffalo – cows during oestrus phases.