In a trial to investigate the role of Pasteurella multocida in disease problems in ostrich farms at different provinces in Egypt. Twenty two cases are obtained and 154 samples from these cases (liver, heart, spleen, lung, trachea and Bone marrow) beside Nasal cleft swab from diseased birds. The clinical signs revealed high morbidity and mortality with respiratory distress and gastroenteric findings. The post mortem findings revealed a picture of hemorrhagic septicemia with enteritis. Bacteriological Examination revealed isolation of 42 isolates from 154 samples with a percentage of 27.3%. The serotyping of the isolated strains revealed that they belong to serotypes 5: A, 8: A, 9: A and 2: D while 12 isolated strains were untyped. All the isolated strains were highly pathogenic for mice and chickens. The antibiogram test revealed that highly sensitive to ciprofloxacin, trimethoprim, colistin sulphate and doxycycline and resistant for penicillin, lincomycin. The pathological changes in different internal organs revealed highly pathological changes. It could be concluded that Pasteurella multocida is highly pathogenic for ostriches and cause severe losses in ostrich farms and found that the rate of isolation of Pasterulla multocida is very high in most bacterial agents. Therefore biosecurity measures must be completely used to minimize the predisposing factors with strict hygienic measures at the farm slaughter house and back yard of ostrich farms.