A total number of 395 milk samples were collected from apparently healthy she-goats and ewes at different localities in Assiut Governorate, comprising 197 halves of milk samples from 100 lactating she-goats and 198 halves of milk samples from 100 lactating ewes. Our current results showed that 34.52 & 9.60% and 34.01 & 9.60% were positive in the examined she-goats and ewes halves by using California Mastitis Test                          (CMT) and Modified Whiteside Test (MWST), respectively. While, out of tested animals 44 and 14% were positive for each test, respectively.  Moreover, screening tests used for detection of Subclinical Mastitis          (SCM), one-half and two-halves of she-goats and ewes were affected in percentages of 20 & 24 and 9 & 5%, respectively. Furthermore, out of she-goats halves examined by bacteriological and mycological examinations, 15.23 and 1.02% were positive, while in case of ewe's halves, 8.08 and 0% were positive, respectively. Concerning 100 of each she-goats and ewes animals examined bacteriologically, 27 and 12% were positive, respectively. While in case of mycological examination, 2% were positive in she-goats however, ewes gave negative results. The incidences of the isolated bacteria causing SCM in she-goat's milk samples were 6.60, 6.09, 1.02, 1.02, 1.02 and 4.06% for Staph. aureus, coagulase negative Staphylococci (CNS), Str. pyogenes, Str. agalactiae, E. coli and Ent. spp., respectively. Whereas, in ewe's milk samples they were 4.04, 4.55, 0.51, 1.01, 0.51, 0.51 and 1.52% for Staph. aureus, CNS, Str. pyogenes, Str. agalactiae, E. coli, Klebsiella pneumoniae and Ent. spp., respectively. The incidence of the isolated molds in she-goat's milk samples was 0.51% each for Aspergillus fumigatus and  A. terreus. Recommendations were suggested to eradicate and control SCM in lactating goats and sheep.