Chemical and microbiological analysis were carried out on 75 raw milk samples randomly collected from some dairy farms of Assiut city to evaluate the sanitary condition of raw milk consumed in Assiut. The keeping quality tests which depend on increase amount of acidity revealed an average 6.8 with the pH value and 0.16% as acid percentage with the titrable acidity.  Clot on boiling and Alcohol precipitation tests scored 36 and 41.3% respectively. The results of keeping quality tests depend on increase amount of enzymes recorded 10 good samples (13.3%), 8 fair (24%), 21 bad (28%) and 26 very bad samples (34.7%), with resazurine test. The amount of free oxygen in the simple catalase tube test was 0-2 in 12 good samples (16%), 2-5 in 24 fair samples (32%) and was bad in 39 samples (52%) more than 5cc free O₂.  In case of microbiological analysis the averages of total bacterial, coliform, yeast and mould counts were 4x10⁶, 9x10³, 12x10³ and 3x10³/ml respectively. Examination for anaerobic spore- formers detected 25.3% of the examined samples. The public health importance of the counted organisms and the prophylactic measures to improve the quality of dairy farm milk were discussed.