A total of 250 waterfowl (150 ducks and 100 geese), having a history of respiratory disorders and mortality, additional to 30 water and 30 sediments samples which waterfowl income were examined in the present study. Samples were collected from different private farms and flock's ducks and geese in Alexandria Province for P.M and bacteriological examination. The clinical sings showed exhausted birds, drowsy, loss of appetite, swim in circles, lameness, swollen wattles, difficult breathing, cyanosis, watery green yellowish diarrhea, ruffled feathers, mucous discharge from the mouth, while postmortem lesions revealed hemorrhages of various sizes on the heart, liver, gizzard and intestines, also white spots and necrotic foci were present on the liver and spleen. Bacteriological examination of these samples for the prevalence of P. multocida according to morphological characters and biochemical reactions, revealed 134 P. mulotcida isolates out of 360 samples with incidence of (43.2%). 92(61.3%) of P. mulotcida isolates from ducks, 36 (36%) and geese samples, 4 (13.3%) isolates from water samples and 2 (6.7%) isolates from sediments samples. There are variations in the prevalence rate of P. multocida isolates from apparently healthy, diseased and freshly dead in both ducks and geese examined. It was found 3 (7.5%) in apparent healthy, 75 (53.6%) in diseased birds, while was 50 (71.4%) in dead birds. Results of experimental infection of P. multocida isolates in mice and ducks revealed difference in mortality rate between strains isolated which ranging from 20% to 100% due to difference in the virulence of these strains. The clinical symptoms and post-mortem pictures of experimentally inoculated ducks are similarly to those observed in naturally. Sensitivity test revealed that P. multocida isolates were more sensitive to Penicillin, Lincomycin and Oxytetracycline, while were resistant to Chloramephenicol, Neomycin and Streptomycin.