For a meaningful diagnostic clinical report of abnormality in the camel, there must be a standard of familiar reference values regarding the management and environmental factors in which the animal acclimatized. The aim of the present study was to give an idea on the values of macro-mineral in healthy dromedary camels in the New-Valley area. A total number of 160 apparently healthy camels were selected from their natural habitat in rural areas of El-Kharga oasis and equally classified according to their sex, age, season and reproductive status. Macro-minerals were determined in the grasses browsed by camels and blood serum of these camels. Results showed that Ca concentration in the pasture was marginal whereas P concentration was deficient than the requirement of camels. The recorded over all-mean values of macro-elements (mmol/l) in serum of these camels (and ranges) were 2.044 ±0.051 (1.2-3.1) for Ca, 1.483 ±0.032 (0.8-2.1) for P, 0.984 ±0.022 (0.6-1.5) for Mg, 151.9 ±1.299 (125-182) for Na, 5.16 ±0.094 (3.2-7.3) for K and 98.6 ±1.119 (76-123) for Cl. These concentrations were recorded for healthy camels and can be used as indicative values. The values of Ca and P decreased with increasing age (P<0.05), females had lower mean values than males (P<0.01) and also the values decreased in pregnancy and lactation (P<0.05). On the other hand, the mean values of Na and Cl were higher in summer than in winter season (P<0.01) and decreased (P<0.05) by lactation. The mean values of Mg and K in serum were not significantly affected by the physiological or environmental status. The present investigations show marked differences in the concentration of serum macro-minerals between camels when retained on similar pastures which may be attributed age, sex, productive and reproductive status of the animal as well as the environmental conditions.