This work was undertaken to investigate gender-related variations in normal serochemical values of German Shepherd dogs in the Sudan and to establish overall reference values for these parameters in this breed. The studied animals were 22 males and 22 females. Serum concentration of total protein, albumin, glucose, cholesterol, triglycerides, creatinine, urea, uric acid, and the activity of the enzymes ALT, ALP and AST were tested. Gender had no significant effect on the studied parameters, except of the activity of the enzyme AST. The overall means and range of the investigated values were calculated, compared and discussed with other researchers results in dogs.