Bovine parainfluenza virus Type 3 (BPI3) and bovine respiratory syncytial virus (BRSV), are ubiquitous respiratory pathogens of cattle, which contribute to causation of bovine respiratory disease complex. As these respiratory viral pathogens cause very similar clinical signs, laboratory diagnosis of these pathogens becomes important. A total of 18 respiratory samples (11 nasal swab samples collected from diseased calves and 7 lung tissue specimens from dead calves) were collected from a farm in Kafer El-Sheikh Governorate. All specimens were analyzed for BPI-3 by heamadsorption and direct immunofluorescent techniques. The same samples were investigated for the presence of BRSV using indirect fluorescent antibody technique and ELISA. RT-PCR technique was used to confirm the presence of both viruses. BPI-3 virus was detected by RT-PCR assay in 17 of 18 (94.4%) samples tested versus to 13 of 18 (72.2%) were detected by FAT. Also, BRSV was detected by RT-PCR in 16 of 18 (88.9%) specimens tested versus to     14 of 18 (77.8%) were detected by ELISA technique. Considering the higher sensitivity of RT-PCR assay that revealed from previous data, it can be recommended as the method of choice in diagnosis of both BPI-3 and BRSV.