Mycoplasma bovis (M. bovis) induces various clinical manifestations in cattle, such as mastitis, arthritis, and pneumonia. A latex agglutination test (LAT) has been developed to detect antibodies in bovine sera as a field test. A total of 80 milk and serum samples collected from a confirmed infected bovine farm were tested by conventional cultural method and indirect enzyme linked immunosorbant assay (ELISA). Milk samples cultivation revealed positive in only 24 (30%) while ELISA detected 33 (41.25%). LAT and ELISA on serum samples detected only 54 (67.5%) of samples. LAT allows rapid and inexpensive test that could be useful in the field for primary herd screening prior to confirmatory laboratory diagnosis of M. bovis.