In this study, a total of 125 samples of raw cow, sheep, camel milk, and ice cream were collected from different sources in Qena Governorate and were analysed for the presence of Staphylococcus aureus. There were 31 S. aureus isolates could be detected from these samples, which were investigated for antibiotic resistance. The obtained results indicated that S. aureus was isolated from (24%) of cows' milk samples, (13.3%) of sheep milk samples, 20% from the camel milk samples and (40%) of ice cream samples. Resistance of S. aureus to different antibiotics was determined by the disc diffusion method. There was a high rate of resistance to penicillin (96.77%), dicloxacillin (80.64%), ampicillin and erythromycin (74.19%) was frequent for S. aureus strains. A low prevalence of gentamycin resistance (9.67%) was detected in (28) S. aureus strains. The public health hazard of Staphylococcus species and the sanitary measures for improving milk quality were recommended.