Listeria infections caused by microorganisms of the genus Listeria, occur worldwide and in a variety of animals and man. Listeriosis was recognized as a food –borne human disease that prompted intense research activity. Thus a total of 100 samples including  (50) raw cow's milk  and (50) of both ice cream small and large scale producers purchased  from retail local markets , supermarkets and street vendors in Qena city. These samples examined to investigate the occurrence of listeria species. Listeria spp < em>. could be detected in 15(30%) and 18(72%) of examined raw milk and small scale ice cream samples respectively, while failed to be detected in large scale ice cream samples.                   L. welshimeri  was identified in  one sample (2%) in raw  milk, and 3 (12%) in small scale ice cream, while L. inoccu present in 2 samples (4%) of raw  milk, and in 3 samples (12%) of small scale ice cream.       L. murray was isolated from 4 samples (8%) of raw milk and 4 samples (16%) of small scale ice cream. L. gray was detected in 5 samples (10%) of raw cow's milk and 6 samples (24%) of small scale ice cream. Suspected L. monocytogenes has been recovered in 3 samples (6%) of raw milk and L. seeligeri was detected in 2(8%) of small scale ice cream. The public health importance and the recommended sanitary measures were discussed.