Enterobacter sakazakii has been implicated to be a rare, but life-threatening cause of outbreaks and sporadic cases of neonatal infections, particularly in premature underweight babies. The prevalence of           E. sakazakii in powdered and hydrated infant formula milk (IFM) collected from Egyptian pharmacies and hospitals in Ismailia City was investigated using FDA enrichment procedure. A total of 64 samples (26 powdered + 38 hydrated) were tested. The organism was isolated from 2 powdered (7.7%) and 8 hydrated (21.05%) samples. The organism grew rapidly at 35ºC in which its population increased from 3.08 logcfu ml^-1 at 0 time to 3.75, 4.41, 5.04, 6.82, and 8.34 logcfu ml^-1 after 2, 4, 6, 12, and 24 hours, respectively. At 22ºC, E. sakazakii increased from 3.08 logcfu ml^-1 at 0 time to 3.26, 3.60, 4.00, 5.08, and 5.90 logcfu ml^-1 after 2, 4, 6, 12, and 24 hours, respectively. It showed no growth at 4ºC through the first 12 hours and very slow growth along the whole period reaching only 3.3 logcfu ml^-1 by the end of the 24 hour keeping period. Upon investigating the killing effect of microwave heating, it was proved to be a convenient and fast method to reduce E. sakazakii counts in mild, moderate and massively contaminated IFM samples. However, care should be taken to ensure that milk is adequately cooled to the required temperature before being fed to an infant.