The current study was conducted to produce buffalo embryos in vitro from vitrified immature and mature oocytes. Cumulus oocytes complexes (COCs) were obtained from ovaries which collected from a local abattoir. Good quality COCs (immature oocytes) were vitrified immediately after collection. After 22-24 hours of COCs culture, mature oocytes were vitrified similarly. All oocytes (immature and mature) were exposed to vitrification solution of different concentrations (glycerol: 3.3, 6.6 and 10%) at 5, 5 and 10 minutes intervals respectively. The oocytes were finally placed in 10% glycerol and immediately loaded in 0.25 ml plastic straws, each containing 100 µl of 10% sucrose in Modified phosphate buffer saline (M-PBS). The straws were stored in liquid nitrogen for 4-6 months. The straws were thawed in water at 37°C for 30 seconds. After thawing, immature oocytes have been exposed to further maturation for 22-24 h. Mature oocytes were fertilized with frozen-thawed semen capacitated in modified fertilization TALP medium. After insemination, the oocytes were cultured for 6-7 days and the frequency of morula and/or blastocyst was recorded. Data analysis revealed that, the proportion of buffalo oocytes found to be normal after vitrification-thawing procedures was significantly different between immature and mature oocytes (55.4% and 70.6% respectively) and a significantly higher (p < 0.05) percentage of embryos development (morula and blastocyst) was derived from vitrified mature buffalo oocytes than those obtained from immature ones (10.3% Vs. 2.6%).