Subsequent nucleotide sequencing analysis is described to characterize turkey rhinotracheitis virus (TRTV) field isolates and vaccine strains. Five isolates of avian metapneumovirus (aMPV) were isolated from field cases in turkeys with clinical signs of rhinotracheitis. Four out of the five isolated strains were obtained from vaccinated turkey farms from 3 Egyptian governorates (Cairo, Giza and Beni-suif). These field isolates of aMPV were amplified by RT-PCR for the fusion (F) gene. A 315 base pair fragment of PCR product was detected by ethidium bromide staining in agarose gel. The amplified fragments were sequenced and compared with other field and vaccine strains. Phylogenetic analysis of field and vaccine strains revealed that the 5 isolates were closely related to each other and very close to field isolates from type B European strains and distinct from the vaccinal strains used. The data from this work revealed that sequence information helps us understand the evolution and dissemination of viruses and spot the light to the currently used vaccines against TRT in turkey farms.