Fifty samples of chilled Mugil cephalus “Mullet" were randomly purchased from Port-Said markets. The samples were examined for enumeration and isolation of hydrogen sulfide producing psychrophilic bacteria. The incidence of positive samples for hydrogen sulfide producing psychrophilic bacteria was 100% (50). The mean values of the total psychrophilic bacterial counts and hydrogen sulfide producing psychrophilic bacterial counts were 2.1 X 10⁵ ± 2.9 X 10⁴ and 1.8 X 10³ ± 3.3 X 10² CFU/g of chilled Mugil cephalus “Mullet" respectively. The incidence of hydrogen sulfide producing psychrophilic bacterial colony in compared to the total psychrophilic bacteria was 0.86%. The hydrogen sulfide producing psychrophilic bacterial isolates in the examined samples were identified as Pseudomonas spp., Pseudomonas fluorescens and Shewanella putrefaciens with an incidence of 77.78%, 14.44% and 7.78% respectively. The effect of the hydrogen sulfide producing psychrophilic bacteria on the public health was discussed.