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STUDIES ON THE EFFECT OF ADDITION OF KOREAN RED GINSENG® AQUEOUS EXTRACTS ON THE FERTILIZING CAPACITY OF RABBIT SEMEN

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Last updated: 28 Dec 2024

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Abstract

  One hundred sixty eight sexually mature Baladi Black (BB) rabbits (26 bucks aged 9 months and 142 monoparous does in two sequence parities) were used in the present study. Semen was collected artificially, pooled and diluted with sodium citrate diluent. Aqueous extracts of Korean red ginseng® were added to the diluted semen at different concentrations of 0 (control), 25, 50, and 75 µl/ ml, then the diluted semen was divided into two parts, the first part was stored at refrigeration temperature (4 - 6 oC) for up to 3 days while the second part was incubated at 37 oC for up to 4 hrs. Percentages of sperm motility; alive and normal spermatozoa and acrosomal defects were recorded at the different stages of the preservation. Sperm penetration into estrous cow cervical mucus was estimated during diluted semen incubation. Evaluation of the activities of glutamic-oxaloacetic transaminase (GOT); glutamic –pyruvic transaminase (GPT); acid phosphatase (ACP) and alkaline phosphatase (ALP) enzymes in the BB rabbit semen were measured during preservation. One hundred and forty two rabbit does in two consecutive parities were artificially inseminated using either the diluted semen free or supplemented with 50 µl Korean red ginseng extract/ 1 ml diluted semen (the level which showed the ideal effects on semen quality) to estimate the fertility traits. In both parts of the supplemented diluted rabbit semen with Korean red ginseng® extract showed that there was significant improvement in:     (P ≤ 0.05) sperm motility, the percentages of alive spermatozoa, normal spermatozoa and storageability, while the acrosomal defects percentages were significantly (P ≤ 0.05) decreased. Sperm penetration into estrous cow cervical mucus were arranged significantly (P ≤ 0.05) in descending order as obtained by supplemented diluted semen with Korean red ginseng® extract (50, 75, 25 µl/ ml and the control ones respectively). Also there were significant (P 0.05) decreases in the mean values of each of GOT, GPT, ACP and ALP either during preservation at refrigeration conditions or at incubation temperature. Conception rate, Kindling rate  and litter size values were significantly (P ≤ 0.05) better in rabbit does inseminated artificially by using diluted semen supplemented with 50 µl/ ml aqueous extracts of Korean red ginseng® than those inseminated using extended semen  free- Korean red ginseng extract. In conclusion, addition of Korean red ginseng® on diluted rabbit semen might improve the semen quality during preservation at different temperatures and fertility traits of rabbit does inseminated artificially. It was found that the ideal level was the addition of   Korean red ginseng aqueous extracts with 50 µl / 1 ml diluted semen.  

DOI

10.21608/avmj.2009.175009

Keywords

Key words: Korean red ginseng®, semen preservation, Enzymes, A.I, fertility

Authors

First Name

ROWIDA

Last Name

M. RIAD

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Affiliation

Animal Reproduction Research Institute, Agricultural Research Centre, El-Haram, Giza, Egypt.

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First Name

T.S.T.

Last Name

SELEEM

MiddleName

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Affiliation

Animal Production Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt

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Orcid

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First Name

DALAL

Last Name

S. MOHAMED

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-

Affiliation

Animal Reproduction Research Institute, Agricultural Research Centre, El-Haram, Giza, Egypt.

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Volume

55

Article Issue

123

Related Issue

24381

Issue Date

2009-10-01

Receive Date

2009-09-10

Publish Date

2009-10-03

Page Start

1

Page End

16

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_175009.html

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https://avmj.journals.ekb.eg/service?article_code=175009

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24

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

STUDIES ON THE EFFECT OF ADDITION OF KOREAN RED GINSENG® AQUEOUS EXTRACTS ON THE FERTILIZING CAPACITY OF RABBIT SEMEN

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Article

Created At

23 Jan 2023