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174989

NEUROPROTECTIVE EFFECT OF GREEN TEA EXTRACT AGAINST LEAD TOXICITY IN RATS

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Last updated: 23 Jan 2023

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Abstract

The toxicity of lead (Pb) is of concern to public health due to its persistence in the environment. Brain is one of the major target organs where severe neurological alternations may be triggered after exposure. Pb could disrupt prooxidant/antioxidant balance of tissue which leads to physiological dysfunction. Green tea extract (GTE) is antioxidant, free radicals scavenger and has chelating property. This study was conducted to investigate effect of GTE on reducing Pb toxicity of the brain of male rats. Four groups of male rats (each 15 rats) were utilized as following: control, GTE–group (1.5% w/v), Pb–group (0.4% lead acetate in distilled water), Pb + GTE-group. Rats received GTE and/or lead orally in drinking water for 6 weeks. Levels of oxidant and antioxidant [lipid peroxides (LPO), nitric oxides (NO), total antioxidant capacity (TAC), glutathione (GSH), glutathione S-transferase (GST), superoxide dismutase (SOD)] were measured using colorimetric methods. Pb concentration in brain tissue was measured by atomic absorption spectrometer. Histological sections of brain tissues were prepared and examined using the routine pathological technique. Pb concentrations in Pb-treated group were higher in brain tissue than controls. In Pb-group, levels of LPO were higher while, NO and GSH were lower in plasma than controls. Plasma level of TAC was lower in Pb- treated group than control. Levels of SOD; GST in tissue were lower in Pb-exposed rats versus control. GTE co-administrated with Pb appeared more effective in reduction of Pb contents, LPO and increase antioxidant status in plasma and brain tissue comparing to Pb-group. Also, severe destructive changes were observed in the brain tissue, treated with Pb alone, represented by meningitis, neuronal degeneration, cerebral infarction, interphase encephalitis and purkinje cell layer degeneration and necrosis in the cerebellum. While the cases treated with lead and GTE, showed marked improvement in the cellular structure of the brain, represented by vacuolated neurons and absence of necrosis of the cerebellar purkinje cell layer. The data from this study suggest that lead can induce toxicity by interfering with delicate balance between pro- and antioxidants. The treatment of rats with GTE combined with Pb could enhance antioxidant/ detoxification system which consequently reduced oxidative stress and Pb burden in the brain thus potentially reducing Pb toxicity and tissue damage. Running title: Lead toxicity: Effect of Green Tea Extract on Oxidative Stress in Rat Brain.  

DOI

10.21608/avmj.2009.174989

Keywords

Keywords: Rat, Brain, lead toxicity, Green tea extract, Oxidative Stress

Authors

First Name

E.E.

Last Name

MEHANA

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Affiliation

Dept. of Pathology, Faculty of Veterinary Medicine, Alexandria University

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First Name

A-R. M.A.

Last Name

MEKI

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Affiliation

Dept. of Biochemistry, Faculty of Medicine, Assuit University

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Volume

55

Article Issue

123

Related Issue

24381

Issue Date

2009-10-01

Receive Date

2009-09-10

Publish Date

2009-10-03

Page Start

1

Page End

20

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_174989.html

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https://avmj.journals.ekb.eg/service?article_code=174989

Order

18

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023