This study was conducted on buffalo herd (130 animals, Janakleez district, Behaira Province) showing a red hair and skin, allopacia in newly born buffalo calves ageing from one day up to 15 days old. Clinical examination of the animals showed that the majority of buffaloes in the herd had rough hair coat, loss of hair pigmentation in addition to curled toe in some adult buffaloes). All the animals were sluggish in movement and had low fertility. The mean serum copper level recorded was 2.93±0.78 μmol/L in buffalo calves while serum dams revealed a mean value of 5.23±0.48 μmol/L. Copper level in both neonates and their dams indicates a severe deficiency. The obtained results drew the attention for the importance of periodic analysis for serum, soil, and ration to avoid deficiency complications of trace elements early