Foodborne diseases are a widely spreading and growing public health problem, in both developed and developing countries. Salmonella microorganisms are one of the most important causative agents of food poisoning, so this study was planned to determine the incidence of Salmonella enteritidis in 546 samples from different sources of animal origin as live poultry, eggs, poultry meat, beef meat and milk products, and also of human origin as food handlers and patients suffering from food poisoning. Detection of Salmonella enteritidis antibodies in patient serum was detected by ELISA .Fourteen strains (14%) of S.enteritidis were isolated from 100 samples of live poultry, 2 strains (2%) from 100 samples frozen poultry, 1 strain (1%) from 100 samples frozen meat, 1 strain (2%) from 50 egg samples, 3 strains (12%) from 25 unpasteurized raw milk samples, 2 strains (8%)from 25 home made ice-cream samples, 6 strains (12.5%) from 48 stool samples of food poisoning people and 4 strains (13.3%) from food handlers. The results ofELISA proved that 8 (16.67%) of 48 serum samples had S.enteritidis antibodies.