A total number of 143 cows and buffaloes cases in Qena and                El-Menoufya Governorates were examined for bovine tuberculosis using single intradermal cervical tuberculin test (SICTT). 109 out of 143 (76.22%) tested cattle were found positive to tuberculin test. Tissue samples from organs positive cases to tuberculin test were examined microscopically with Ziehl-neelsen stain and by cultivation on two different types of media (Middlebrook and Lowenstein Jenssen media). Blood serum samples from the positive cases were tested by ELISA. ELISA plates were coated with bovine purified protein derivative (PPD) and short term culture filtrate (ST-CF) antigens. The test sensitivity was compared at different serum dilutions. The result was102 out of 109  cases were positive by cultivation technique with a percentage of (71.32%) and 94 out of 109 cases were positive by microscopical examination with a percentage of (65.73%). The results of ELISA and traditional cultural methods as well as tuberculin test were compared and discussed. All serum samples of tuberculin positive cow cases were tested by indirect ELISA, and showed that 26 out of 31 cow sera were positive by PPD with a percentage of (83.87%) and 29 out of 31 cases were positive by using ST- CF antigens with a percentage of (93.5%) in (1\40) serum dilution. Concerning buffalo cases, 71 out of 78 tuberculin positive sera were positive with PPD antigen with a percentage of (91%) and 77 sera cases were positive with ST- CF antigen with a percentage of (98.7%) at serum dilution (1\40). Regarding the tuberculin negative cases, out of 16 tuberculin negative cow cases there were 9 positive cases by using PPD antigen with a percentage of (56.25%) and 6 positive cases by using ST- CF antigen with a percentage of (37.5%). Concerning the buffalo cases, there were 3 positive cases out of 18 tuberculin negative sera by using PPD antigen with a percentage of (16.66%) and one case was positive by using ST- CF antigen with a percentage of (5.55%).