The aim of this study was to investigate the changes in CPK, LDH, AST, ALT, ALP and GGT activities during late pregnancy, parturition and early lactation in 5-15 years old 18 pregnant thoroughbred Arabian race mare. Blood samples were collected from jugular vein on days 45, 30 and 15 before parturition, at parturition and on days 3, 10, 15, 22, 28, 35 and 45 after parturition. Serum CPK levels significantly decreased towards parturition (p < 0.05). Serum LDH activities rose on days 35 and 45 of lactation period. Serum AST and ALT levels showed significant increases on day 30 before parturition (p < 0.05). Serum ALT levels showed a significant decrease on day 10 of lactation period (p < 0.05). Serum ALP activities increased at parturition (p < 0.05) and decreased after parturition. Serum GGT levels did not change during the study period. According to the present research results, activities of all enzymes during late pregnancy, parturition and early lactation remained within reference values recorded in different breeds or species. The values obtained in this study may assist clinicians to interpret biochemical data of mare in pregnancy and lactation.