Experimental ventral cystotomy wounds were performed on 72 adult dogs of different sex and age. Cystotomy wound was closed by chromic catgut, polyglycolic acid, vicryl, Polysorb, silk and methyl-2-Cyanoacrylate super glue. Animals were sacrificed at one, two and three weeks postoperatively, and site of operation were examined grossly, microscopically and by scanning electron microscopy. The results of this study reconfirms that catgut is the best thread used for suturing of the urinary bladder incisions. Polyglycolic acid, vicryl and Polysorb were hydrolyzed and they are not sufficient for suitable time for healing of wound. Silk not absorbed and can cause the nidus for urinary calculi. Non absorbable tissue adhesives were unsuitable for urinary bladder closure, because there is no healing at the site of operation and lead to deformity and shrinkage of the bladder wound.