One thousand one hundred fifteen sheep blood serum samples have been collected arbitrary from the prevailed herds in South Syria and that is    according to the breeding density which covered 4 Provinces as following: Damascus, Quoneitra, Daraa and Sueida. Diagnostic serological tests were performed to detect the brucellosis antibodies which showed that 54 samples were positive for Fast agglutination test and the entire prevalence rate was (4.84%). Whereas 46 samples were positive for the Complement Fixation Test which is considered the most important test to assure the         existence of brucellosis antibodies, and the entire rate was (4.12%). These   results were discussed according to the study data with a trial to find     successful monitoring in order to put a plan to control and eradicate this disease.