This study was undertaken to determine the values of some physiochemical parameters of cerebrospinal fluid (CSF) of healthy Sudanese steers and compare them with their corresponding serum values. The experimental animals were thirty clinically normal steers presented to the Slaughter House of The Animal Production Research Centre at Kuku.           Cerebrospinal fluid samples were taken from the cisterna magna and blood was withdrawn from the jugular vein. Some electrolytes, metabolites  and enzymes activities were determined in cerebrospinal fluid and serum. The mean values for CSF constituents were as follows; total protein(TP) (6.45±0.03mg/100ml), albumin  (2.57±0.02mg/100ml), urea (17.97±0.25mg/100ml), Creatinine (1.17±0.021mg/100ml), glucose(47.67±0.26   mg/100ml), Na(104.87±1.57mmol/L), K(3.09±0.03mmol/L), Ca(5.48±0.03mmolL), and Mg (1.49±0.03mg/100ml) ,and the activities of the enzymes ALP (75.10 ±0.51 U/L), AST(20.13±0.37 U/L) and ALT(5.50±0.18 U/L) and their corresponding values in serum were (7920±0.03mg/100ml)(3920±0.03mg/100ml);(27.27±0.26mg/100ml); (1.36±0.02g/ml);(48.18±0.28mg/100ml);( 113.20±0.99 mmol/L);( 3. 66±0.03 mmol/L) ;( 7. 72±0.02 mmol/L) ;( 47.57±0.48 µg/100ml) , (1.28±0.02mg/100ml);( 84.08±0.29 U/L);( 30.73±0.61 U/L) and (10.17±0.44 U/L) respectively. The mean vlues of all the studied parameters, except of Mg, were significantly lower in CSF than those in serum. The obtained results were discussed with previous reports in cattle and other domestic animals. The values reported in this study can add to the reference value for Sudanese cattle.