This study was carried out on 86 buffaloes from a private farms at Dakahlia Governorate to investigate the bacterial causes associated with oedematous skin disease. Bacteriological examination of the samples revealed that 36 cases (41.9%) were positive. 23 cases (63.9%) were in form of the closed lesions and 13 cases (36.1%) were open lesions. The main clinical signs of the infected buffaloes had been described. Isolated bacteria were subjected for morphological and biochemical identification. The results obtained revealed that 30 isolates (83.3%) were Corynebacterium pseudotuberculosis in single infection and mixed infection with both Staph. aureus 4 isolates (11.1%) and Strept. pyogenes 2 isolates (5.6%). Pathogenicity test for C. pseudotuberculosis in guinea pigs indicated that all isolates were pathogenic and cause the death of inoculated animals. Results of antibiogram indicated that most of the isolates were highly sensitive to Gentamycin, Pencillin, Ciprofloxacin and Streptomycin. Treatment by effective antibiotics, antihistaminic and eradication of the flies achieved the recovery from the disease.