This study was carried out in a village at Assiut Governorate, Egypt to detect the prevalence of Cryptosporidium parvum in different water samples that affecting the health condition of buffaloes and sheep. A total of 34 different types of water samples and 74 fecal samples from water-buffaloes and sheep were tested for Cryptosporidium parvum oocysts using PCR techniques. Cryptosporidium parvum were detected in local surface water, dug-well and tap-water in percentage of 70 %, 58.33 % & 41, 66 %, respectively, with overall percentage of 55.88%.Moreover, our study showed thatthe overall percentage of Cryptosporidium parvum among buffaloes and sheep in Assiut province, Egypt were 51.77% and 51.11%, respectively. Moreover, the results showed that the percentage of Cryptosporidium parvum oocysts in adult ewes and lambs were 40.00 % and 56.66%, respectively and adult female buffaloes and buffalo's calves were 46.66% and 57.14 %, respectively.