This experiment was designed to determine the effect of supplementing practical broiler diets with some oils: olive oil, soya been oil, fish oil, and linseed oil on total fat, and composition of fatty acids in the breast and thigh muscles. Atotal of 240 one day old broiler chicks were used. They were distributed into eight groups containing 30 chicks each. Eight vegetable diets were prepared, containing different oils: 1- soya been oil 6% (control), 2- olive oil 6%, 3- fish oil 6%, 4- linseed oil 6%, 5- mixture of olive oil 3% and linseed oil 3%, 6- mixture of olive oil 3% and fish oil 3%, 7- mixture of linseed oil 3% and fish oil 3%, 8- mixture of olive oil 2%, fish oil 2% and linseed oil 2%. All diets were composed of yellow corn and soya bean meal (44%), and other supplements and formulated to meet NRC, 1994 requirements. The experiment was lasted for six weeks.Ten birds were slaughtered, thigh and breast samples were collected for fat extraction and fatty acids analysis by gas chromatography. Results showed that male birds fed on diet containing linseed oil showed the highest value ofα-linolenic acid (omega-3), (10.23% and 11.02% respectively) with high significant difference (p < 0.01) in fat of breast and thigh muscles when compared to control birds. Breast and thigh muscle's fat of male birds fed on diet containing olive oil, showed the highest value of oleic acid (49.32% and 55.18% respectively) with high significant difference (p < 0.01)when compared to control birds. Male birds fed on control diet showed highest value of linoleic acid in breast and thigh muscles (35.38% and 37.64% respectively). In female birds, breast and thigh muscle's fat of female birds fed on diet containing linseed oil showed highest value of α-linolenic acid (omega-3), (8.13% and 5.85% respectively) with high significant difference (p < 0.01) when compared to control birds. Breast and thigh muscle's fat of female birds fed on diet containing olive oil, showed highest value of oleic acid (55.68% and 53.59% respectively) with high significant difference (p < 0.01) when compared to control birds. Female birds fed on control diet, showed highest value of linoleic acid in breast and thigh muscles (31.26% and 30.67% respectively).