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173745

EFFECT OF GOAT BREEDS, SEMEN DILUENTS AND FREEZING METHODS ON SPERM FREEZABILITY AND REPRODUCTIVE PERFORMANCE

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Last updated: 05 Jan 2025

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Abstract

Two experiments were designed to study the effect of semen extenders and freezing regimens on post-thaw semen motility and viability index, effect of bucks breed was also included (experiment 1). In experiment 2, semen diluents effects on reproductive performance was conducted. Semen was collected from bucks breeds (Aradi (A), Damascuss (D) and cross (½A½D). Good quality semen was divided into 4 portions, each diluted with one diluent (Milk, Na.Citrate, Tris and Na.Bicarbonate). The diluted semen was packaged into 0.5ml straws then cooled to 5°C. After equilibration, half of the packaged straws were suspended 15cm above liquid nitrogen (LN) for 15min (Freezing regimen1; slow). Other half of straws was suspended at height 15 and 5cm of LN for 10 and 5min, respectively (Freezing regimen2; rapid) before plunged into LN. Frozen semen was thawed for post-thaw motility and viability. In the second experiment, semen with good quality was extended with three types of extenders (Milk, Na.Citrate and Tris). Diluted semen were cooled to 5°C and used for AI. Results revealed that, pre-freeze semen motility was significant higher in Tris, Na.Citrate and Na.Bicarbonate than milk diluent. Post-thaw semen motility and viability were highly significant for milk and Na.Citrate than Tris and Na.Bicarbonate diluents. Post-thaw semen motility was significantly higher in Aradi and Damascus than cross breed. Post-thaw semen motility and viability revealed, significant higher means for Freezing regimen 2 than regimen 1. Milk was significantly higher than Tris and Na.Citrate diluents for fertility and fecundity. It was concluded that, regarding to post-thaw semen viability, fertility%, kidding%, fecundity% and prolificacy%, milk is preferable than Tris and Na.citrate diluents.

DOI

10.21608/avmj.2012.173745

Keywords

Key words: Buck, semen diluents, artificial insemination, fertility

Authors

First Name

MOHAMMED,

Last Name

KM.

MiddleName

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Affiliation

Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), Egypt

Email

kamel14@hotmail.com

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Orcid

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First Name

M.H.

Last Name

KHALIL

MiddleName

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Affiliation

Faculty of Agriculture, Benha University, Moshtohor, zip code 13736 Kalyoubia, Egypt

Email

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City

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Orcid

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First Name

A.M.

Last Name

AL-SAEF

MiddleName

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Affiliation

College of Agriculture & Veterinary Medicine, Qassim University, Saudi Arabia

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Volume

58

Article Issue

133

Related Issue

24335

Issue Date

2012-04-01

Receive Date

2012-03-29

Publish Date

2012-04-01

Page Start

1

Page End

12

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_173745.html

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https://avmj.journals.ekb.eg/service?article_code=173745

Order

27

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

EFFECT OF GOAT BREEDS, SEMEN DILUENTS AND FREEZING METHODS ON SPERM FREEZABILITY AND REPRODUCTIVE PERFORMANCE

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Article

Created At

23 Jan 2023