The study was conducted on one hundred and ten adult sheep from private farms in different localities in Ismailia Governorate. Which Ninty sheep were naturally infected with Babesia ovis and Twenty sheep were proved by clinical and laboratory method to be healthy and used as control. The clinical signs of diseased sheep revealed a marked hyperthermia, loss of appetite, depression, weakness, cessation of rumination, extreme pale mucus membrane due to severe anaemia, increased respiratory rate and labored breathing. The urine was dark red to brown coloration and later on jaundice subsequently developed. The biochemical data reveled that a highly significant increase of both serum sialic acid and adenosine deaminase activity in infected sheep in comparison with healthy one. In addition total bilirubin, direct and indirect levels were highly significant increase in infected sheep when compared with the healthy one. Total protein was significantly decreased with hypo-albuminaemia, while globulin, glucose and cholesterol were non-significantly increased. Blood serum calcium and sodium levels showed a significant decreased, while serum level of inorganic phosphorus was significantly increased in infected animals. Blood serum iron revealed highly significant elevation, while copper and zinc levels showed highly significant decreased. Also significant increase in AST, ALT and AP were evident in infected sheep comparatively with control.