A total of 200 random samples of milk from dairy shops, farms and markets (100 farm's milk samples from Cairo, 50  milk samples  from different dairy shop in Port-Said and 50 samples of Ultra heat Treatment (UHT) milk from different Port-Said city markets) for isolation and identification of Staph aureus, Salmonella speciesand Listeria monocytogenes. The results revealed that the staph aureus could be detected in 9% and 28% of farm's and dairy shops milk samples, respectively, with count values 3.6 x10² and5.2x10², respectively. Out of 14 strains obtained from dairy shops milk, only two were enterotoxigenic belonging to type A&D and C. Nine Staph aureus were obtained from farm's milk, one was enterotoxigenic belonging to type A. While, Salmonella speciesand Listeria monocytogenes wereisolated fromfarm's milk only in percent of 5% and 4 %, respectively. The isolated Salmonella serotypes were Salmonella anatum (one isolate) and Salmonella typhimurium (four isolates). On the other hand the Ultra Heat Treatment (UHT) milk from different markets were free from Staph aureus, Salmonella speciesand Listeria monocytogenes. The public health hazard of these microorganisms as well as recommended measures to improve quality status of milk were discussed.