In order to investigate seroprevalence Bovine Herpesvirus-1- antibodies in dairy cattle farms in Syria , 230 serum samples were collected from cattle. The age , sex and relationship with percentage of infection with Bovine herpesvirus-1- were studied by using Competitive Enzyme Linked Immunosorbant assay (cELISA) and Neutralization test(NT).The total percentage of seropostive cattle was 45.21% by using cELISA ,while the lowest percentage was recorded  36.09% by using NT . The results indicated the high infection were found in Gub Ramla 60% ,while the lowest percentage was detected in Zerba 30% by using both tests . Results showed high percentage of acute infection in the age less than 6 months to both sex, while the high percentage of chronic infection recorded in female (2<- 4 years) .No chronic infection recorded in young both sex calves .High percentage of acute infection detected in Jourin , while high percentage of chronic infection recorded in Gub Ramla .Comparison between the two tests used in this study , Showed that high percentages of infection in cELISA ,while in NT recorded less percentages. The high percentage of infection appear in cattle aborted in 2^nd trimester of pregnancy when compared  with other ones , high percentage were recorded in cattle which suffer from reproductive disorders . The high percentage of infection with Bovine herpesvirus-1- recorded in animals which suffer from respiratory signs ,while the lowest infection rate recorded in healthy one ,without any significant of variance.