Mastitis is one of the most expensive disease of milky cows, fungi, especially yeasts can be important etiological factor of mastitis. The aim of this work was to determine the frequency of yeasts isolation from milk of cows suffering from mastitis. A total of 80 milk samples from cows suffering from mastitis were examined. Yeasts were isolated from 50(62.5%) milk samples but 26(32.5%) samples were positive for other fungi (Aspragillus flavus, Aspragillus niger, Pencillium and Mucor) and only 4(5%) samples were negative for fungi isolation. About 20(40%) samples were pure yeast isolation, the other 30(60%) milk samples were mixed yeast and other fungi. Only 20(40%) strains of yeasts classified as Candida genus. Identification was adopted on the basis of morphological and biochemical characteristics including Germ tube test, urease production and carbohydrates assimilation. From the present study, prevention of mycotic mastitis requires management practices and hygienic management that prevent mycotic growth, advanced diagnosis for identifying yeasts for saving more antibiotics used in treatment. In which the use of antimicrobials for long period is pointed out the occurrance of mycotic mastitis.