Fifty samples of raw cow milk were collected from clinically infected animals from different regions of Mosul city-Iraq, during the period between July to November, 2011 for direct detection of Mycobacterium tuberculosis and Mycobacterium bovis in the milk by using species-specific PCR technique. The Sacace MTB complex kit was used for DNA extraction and amplification from these samples. The Results showed that only four samples from fifty samples of suspected infected milk cow's were positive at ratio (8 %). These results ensured the occurrence of M. tuberculosis and M. bovis in the milk transmitted from animals to human and vice versa which may cause human tuberculosis, the study enhanced the significance of bacterial presence in raw milk and cause the infection to the consumer by drinking the contaminated or unpasteurized milk.