This study was carried out in a private dairy farm at Kaliobeia Governorate to investigate the relationship between preparturient hormonal and biochemical changes in pregnant buffaloes and buffaloes with retained placenta (RP). A total of 32 pregnant buffaloes used in this study, blood samples were collected at late stage of pregnancy (two week, week before calving and after parturition). Preparturient biochemical examination revealed that 25 animals were around normal levels, while blood picture of seven animals revealed anemia with leucocytosis, lymphopenia and monocytosis associated with oxidative stress as shown by obvious increase in nitric oxide (NO), lipid peroxidase (LPO) and decrease of catalase (CAT), superoxidase dismutase (SOD) ascorbic acid (ASCA) and total antioxidant capacity (TAC) values. In addition there were a significant decrease in level of selenium (Se), vitamin E with low zinc (Z), copper (Cu), iron (Fe) concentration. Hormonal changes showed that increase level of progesteron, cortisol while decrease in estradiol-17β. Regular flow up of these animals post parturient revealed that animals considered RP (1-3 days) post calving were subjected to manual removal of there placentae. Therefore this study can put light on prevention of retained placenta is the key to avoid reproductive disturbances. A balanced, limited ration with sufficient amount of antioxidant during the 6-8 week dry period, sufficient daily exercise, sufficiently large clean and comfortable calving areas and proper sanitary procedures during the calving period minimize the chances of retention and infections of reproductive tract.