Edwardsiella ictaluristrains isolated from different fish species in different geographic areas (Japan, Indonesia, Vietnam and USA) were screened against 15antimicrobial agents. The antimicrobial susceptibility was examined using the Minimum Inhibitory Concentration (MIC) test. Except for only one of the tested strains (DTHN01), the otherswere found to be susceptible, with high percentages, to most agents active against gram negative bacteria such as aminoglycosides, β lactams, quinolones, tetracyclines, and chloramphenicol. Resistance was observed against colistin, sulfisozole, Polymixin B sulfate and Vancomycin.