Retained fetal membrane (RFM) is one of the most important calving related disorders affecting economics of the farmer and the herd health. The aim of the study was to compare some hormonal and biochemical parameters level in blood serum of buffalo-cows with retained fetal membranes (RFM) and normal non retained animals (C). Blood samples were collected from buffalo-cows (n=55) for three times (after four months, seven months of gestation and 24 hour after parturition), after parturition only nine buffalo-cows affected with RFM (16.36%). The obtained results, showed significance decrease (P < 0.05) in Calcium, Zinc and Selenium between RFM and C groups after four months of gestation, while, in RFM group after seven months of gestation results revealed significance decrease (P < 0.05) in 17-β Estradiol, calcium and selenium and significance increase (P < 0.05) in progesterone, cholesterol and linolinic free fatty acids. But after 24 hour postpartum there was significance decrease (P < 0.05) in cholesterol, linolinic free fatty acids and calcium, with significant increase (P < 0.05) in progesterone and Cortisol. The obtained results concluded that Progesterone and Cortisol were significantly increased remarkably just after 7th month of pregnancy, in addition to significantly decreased of estradiol 17-β and biochemical profiles extending till 24 hrs after calving.