For sero-biochemical study on brucellosis affected dairy cattle and human contacts for a period from June 2012 to May 2013, a total of 1210 blood samples were collected from dairy cattle in different localities in Dakahlia Governorate, 2378 blood samples in Damietta Governorate and 1600 blood samples in Alexandria Governorate. Another 120 blood samples from humans in contact with dairy cattle was performed. The collected sera were examined by Rose Bengal test (RBT), Buffered acidified plate antigen test (BAPAT) and Tube agglutination tests (TAT). The serological examination revealed that 216 (17.8%), 212 (8.9%) and 189 (11.8%) were positive in Dakahlia, Damietta and Alexandria Governorates respectively and also 20 samples humans contacts were positive. Haematological and biochemical examination of 7 positive samples collected from both infected cattle and diseased human compared with the healthy group revealed a significant (p < 0.01) decrease in TRBCs, Hb concentration and PCV while TWBCs was significantly (p < 0.001) increased. Biochemical estimation revealed a significant decreases in total proteins (p < 0.05), albumin (p < 0.01) and non significant decrease in total globulin. There was a significant (p < 0.01), (p < 0.001) increase in the activities of Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST) in cattle and human respectively. Analysis of some minerals in diseased cattle and humans showed a highly significant (p < 0.01) decrease in calcium and inorganic phosphorus levels and a significant (p < 0.05) decrease in magnesium, iron and sodium levels, besides non significant variations in the potassium level when compared with healthy one. It is thus recommended to the owners of the farms and yards must be harry up for the early diagnosis of this serious zoonotic disease followed by the eradication of the sero-positive animals and vaccination of the negative animals. Moreover therapeutic plan should be cleared for the diseased farm workers. The cooperation between the VeterinaryServicesCenters and Public Health Authorities should be planed for the control of brucellosis in Egypt.