To estimate the levels of some chemical pollutants (nitrite and some heavy metals) in drinking water of some poultry farms in Assiut governorate, ninety representative water samples were collected from different poultry farms at Assiut governorate (Dairut, Elkosya, Mnfalute, Abnob, Elfath, Elbadary, Banygalb, Sahel selem and Abotig). Ten water samples were taken from each localities. Estimation of lead, cadmium and manganese was done by using Graphite atomic absorption spectrometer. Estimation of nitrites was done calorimetrically. There was an increase in the mean levels of lead in water samples collected from poultry farms at all examined areas.  The lead levels in all analyzed water samples were more than the permissible limit recommended by WHO (0.01ppm). Cadmium levels in all examined water samples were below the permissible limit recommended by United States Environmental Protection Agency (US EPA) (0.005ppm). Manganese levels were between the highest desirable level 0.05 mg/1 recommended by US EPA and the maximum permissible level 0.5 mg/I  recommended by WHO. The mean levels of nitrite in water samples  collected from poultry farms of Dairut, Elfath, and Sahel selem were exceed the permissible limit (1 mg/L) recommended by the US EPA. The adverse health effect due to exposure of this pollutants were discussed.