The purpose of this study was to investigate the effect of supplementing in vitro maturation medium and culture medium with insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF) and a combination of them on the development of in vitro–matured and fertilized (IVM/IVF) bovine oocytes, as well as their developmental rate to cleavage and blastocyst stages. For this purpose, 464 bovine ovaries were collected from Beni Suef abattoir; oocytes were aspirated and used for in vitro maturation and subsequent embryo development. The current results revealed that supplementing maturation and culture media with a combination of IGF-I and EGF was the best treatment for improving rates of IVM, IVF, cleavage and blastocyst development. Addition of IGF-I or EGF into maturation medium had insignificant effect on IVM rate but significantly enhanced subsequent embryonic development as compared to control. Moreover, including IGF-I in culture medium significantly improved cleavage, morula and blastocyst rate as compared to EGF-group.