This study was aimed to detection of specific antibodies against Newcastle disease in the egg yolk of different types of chicks and birds, Five samples of eggs were obtained from each of local breed chicks, House breeding (Al-Arab) chicks, ducks, pigeon birds, in addition to hatching birds of broiler, Yolk was separated and diluted in distal water (1:1) and divided in to two parts: First part was used to evaluate the  specific antibodies against Newcastle disease in the egg yolk whereas second part was used as an attemption to elevate levels of protection against ND in small chicks at 28 days old by i.m injection of diluted yolk extract at a dose of 0.5 ml, Thirty chicks classified into six groups was used for this purpose, each group was injected by type of diluted yolk extract of different eggs and last group kept as control group without any treatment, Levels of specific antibodies against ND in egg yolk and serum of injected chicks with diluted yolk were tested by Heamagglutination inhibition test using 4 HA for live attenuated ND virus (lasota). Results were showed that highest titer of specific antibodies against ND was in the yolk of hatching eggs at a level of (32) whereas the lower titer was in the yolk of Duck eggs at a level of (2.6), Whereas the highest titer of injected chicks by yolk extract was in the first group which injected by yolk extract of hatching eggs at a level of (19.2) and lower titer was in group of chicks which injected by yolk extract of Duck egg at a level of (2.2).