In the present study 286 cows with reproductive disorders were serologically tested for natural infection with the most prevalent leptospiraserovars using MAT and ELISA. Estimation of the corresponding serum biochemical changes were carried out also. Serological survey on cows sera showed that leptospiral seropositivity using MAT and ELISA were (45.81%) and (52.10%) respectively. The most prevalent antibodies were detected against L.grippotyphosa, L. Canicola, L.Pomona, L.icterhaemorrhagiae, L.wolfi using MAT (11.89 %, 10.49 %, 10.49 %, 8.55 % and 4.55 %) respectively. And using ELISA (12.94 %, 12.59 %, 11.54 %, 10.14 % and 4.89 %), respectively. The biochemical analysis of cow sera revealed that cows suffered from leptospirosis showed increase in blood urea, creatinine, ALT, AST and GGT.