The present study have been done from September 2013 to February 2014, on 90 horses of both sex and age at three different regions of Nineveh province - Iraq. Blood samples were collected from jugular vein of 90 horses, some animals appeared clinically healthy while others suffered from different clinical signs. Sera were tested for Neopora hughesi antibodies by Recombinant SAG1 Enzyme Linked Immunosorbent Assay ten horses out of 90 (11.11%) reacted positively to N.hughesi antibodies. The animal aged 6-10 years recorded high prevalence and suffered from different clinical signs. The antibodies detected in male were more than that of female without any statistically significant.