A Total of 200 samples (100 cloacal swabs and 100 of eggs from laying farms) were collected in Sohag Governorate. The samples were examined for the presence of Campylobacter. The bacteriological examination revealed isolation of 38   isolates of Campylobacter species from cloacal swabs (25 isolates (65.8%) of C. jejuni and 13 isolates (34.2%) of C. coli). On the other hand one isolate (1.0%) from egg shell was isolated. While trial of isolation from egg content was unsuccessful. Concerning experimental infection of chickens with C. Jejuni, the mortality rate, was (30.0%) among chickens infected intramuscularly, while it was (20.0%) after oral infection .On the other hand the infection with C. coli revealed that mortality rate, was (20.0%) among chickens infected I/M and (10.0%) after oral infection. In vitro sensitivity test, most isolates were highly sensitive to spiramycin, spectinomycin, clindamycin, gentamycin and colistin sulphate and were resistant to amoxicillin and penicillin.