The objective of this study was to investigate the role of administration of gonadotropin-releasing hormone (GnRH) at day 12-14 postpartum on ovarian dynamics and conception rates in buffalo- cows. The study was conducted on twenty four, apparently healthy, lactating buffalo-cows in their second to fifth parity. The animals were divided into two groups; group I (n=10) considered as control group that did not receive any treatment and group II (n=14) that received 100 µg GnRH (Receptal^®) for each animal during the period from 12-14 days post partum. Ovarian structures were monitored daily, from day 6 postpartum until detection of the third postpartum ovulation, using a real- time ultrasound. Blood samples were collected via the coccygeal vein from day 6 post treatment and every 3 days until detecting the third postpartum ovulation to estimate serum progesterone, serum luteinizing hormone, and Follicle stimulating hormone profiles. All animals ovulated before day 45 postpartum in GnRH treated group (14/14,100%); whereas, none of the cows in control group ovulated within this period of time. Interval from calving to the first ovulation and from calving to the second and the third ovulations in GnRH treated group was significantly shorter (23.6±1.4, 45.2±2.3and 67.8±1.9 days respectively) than in control group (65.1±5.8, 87.6±6.4 and 119.2±6.3 days respectively). The mean serum progesterone concentrations were significantly higher in treated group in the first and the second cycle (3.6 ± 0.2 and 4.1 ± 0.1 ng/ml) than in the control group (1.8 ± 0.3 and 3.1 ± 0.6 ng/ml respectively). The pattern of LH release was similar in both treated and control groups, while the peak LH was significantly higher in treated group (21.4 ± 3.8 ng/ml) than the control group (12.8 ± 1.9 ng/ml). The actual intervals from calving to first behavioral estrus and the actual interval from calving to conception (days open) were significantly shorter in treated group (42.6 ± 1.8 days, 86.9 ± 1.6 daysrespectively) than the control group (65.2 ± 3.4, 100.4 ± 2.6 days respectively). The conception rate was higher in treated group (77.4 ± 1.9) compared to control group (55.8 ± 2.6). From the current study we concluded that, GnRH treatment on day 12-14 postpartum stimulate early ovulation in buffalo- cows, thus stimulate the postpartum behavioral estrus, shorten number of days open, decrease number of services per conception and improve the conception rate.